Obviously, sensitivity of the RAPD assay depends of the mutations level and it needs further investigations.
One of the disadvantages of this method is that it does not account for the rate heterogeneity of the different mutations, and that it needs calibration against an archaeological or historical record.
In our study, 11.8% (9/76) of the samples were also found to be both loss of RASSF1A expression and K-ras mutations in CRC; it needs further studies to verify whether inactivation of RASSF1A has synergistic effect with activated K-ras in CRC.
Many scientists believe another one is overdue, and new knowledge of viruses indicates that this strain of bird flu has acquired some of the mutations it would need to be transmitted easily between humans, which has caused much of the alarm this year.
In addition, in order for a mutation to be counted it needed to be present at values above the threshold in both technical replicates.
Hypothetically, the converter domain is sensitive to mutation because it usually needs to perform the two functions of the linear-to-rotary motion converter and elastic element.
Biological relevance of mitochondrial mutations yet needs to be clarified.
Once a risk factor is identified in one subgroup of mutation carriers it would need to be tested across other mutation carriers.
This mutation however needs further evaluation to determine its functional significance.
It is dedicated to characterizing variants in clinical settings where only a limited set of relevant mutations needs to be quickly characterized with the highest possible accuracy.
