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The types of mutations detected are summarised in Table 2 and compared with cII mutations isolated from the livers of riddelliine-treated rats.
Investigate yeast strains that are engineered to express the human tumor suppressor protein, p53, and use modern molecular methods to identify the functional consequences of p53 mutations isolated from tumor cells.
In fact, several recent studies have shown evidence of GAS with covS non-functional mutations isolated from in vivo systemic infections, suggesting that covS mutants posses a selective advantage during invasive infections [48], [54], [56] [61].
Here, we examine the power of BSA-seq for efficiently mapping small effect mutations isolated from a mutagenesis screen.
Interestingly, none of the same mutations isolated from the cardiac tissue were identified in the peripheral blood lymphocytes, indicating the de novo nature of the mutation.
Further biochemical analyses on the structure-function relationship of the mutations isolated from these early genetic screens will provide new information about the Pol II structure, and this emphasizes the mutual benefits that combined genetic and biochemical-structural approaches can provide.
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To verify that the tarsal joint phenotype of fz mutations correlates with the strength of the ommatidial polarity defect, eyes from three mutations isolated in the screen that gave different strength tarsal phenotypes were sectioned.
Both mutations were isolated from humans infected with the virus and were not present in any of more than 600 avian isolates checked, the group reports today in Nature.
The loss-of-function AtTDP mutation was isolated from an Arabidopsis activation T-DNA (transferred DNA) tagging mutant pool as described by Lee et al. [ 19].
The P242S mutation, originally isolated from Drosophila [50], reduces binding to Mint and alters the rate of exocytosis [29], [39].
The 3'-end of spx carrying the DD mutation was isolated from pSN56 digested with BclI and SalI.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com