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Most testing for JAK2 mutations is performed by analyzing the genomic DNA of the JAK2 gene.
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Screening for BRCA1/BRCA2 mutations was performed using denaturing high performance liquid chromatography (dHPLC).
The fraction of the initial population is selected with a probability and then mutations are performed on them.
Mutations were performed in a series of steps.
The DAAM1 N-terminal point mutations were performed using a two-step PCR method.
Mutations were performed with the QuikChange Site-directed Mutagenesis Kit (Stratagene, USA).
Mutations were performed using a QuikChange™ site-directed mutagenesis kit (Stratagene).
Ser27Ala, Ser47Ala and Thr530Ala mutations were performed by using site-directed mutagenesis.
For each of the 100 snapshot structures of the MD simulation, the following in silico mutations were performed.
Site-directed point mutations were performed using QuikChange Mutagenesis Kit according to the manufacturer's instructions (Stratagene, Cedar Creek, TX, USA).
Mutations of the individual GC-box, as well as combined mutations were performed to evaluate the functional significance of these putative Sp binding sites.
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