The mutations are incorporated into the models by substituting the amino acids during structure modeling.
In the double mutants and a triple mutant, the single mutations were incorporated in various combinations.
The mutations were incorporated into wild-type full-length Drosophila Notch (described in [18]) in both the pMT [19] and pUAST [20] vectors.
The importance of the EBox as the primary mediator of the AICAR effect on PGC-1α promoter activity was further evaluated by generating a reporter construct in which mutations were incorporated into the Ebox sequence of the overlapping GATA/EBox sequence within the p851 promoter length.
The resulting pET16b-PAD2 construct was sequenced to ensure that no mutations were incorporated.
All clones were sequenced to confirm that only the intended mutations were incorporated into the constructs.
We verified whether the desired mutations were incorporated by sequencing the TEM locus.
Moreover, we describe two mutations in antigenic sites that may cause antigenic drift in the virus as more mutations are incorporated in these sites.
The mutations were incorporated into E. coli complex I: cluster N1a could no longer be reduced by NADH, but H2O2 and superoxide production were unaffected.
When identical mutations were incorporated into the Envs of either GL8 or C-PGammer, receptor usage was switched from CRD2-dependent to CRD2-independent [ 34 ].
