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Because the assignment of genes to mutations is done based on co-occurrences, sometimes a mutation is assigned to several genes.
DNA testing as a second-tier test to detect high-frequency mutations is done in some programs for CF, hemoglobinopathies, MCAD, LCHAD and galactosemia, and some are considering second-tier testing by MS/MS for CAH.
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All these mutations were done by physically changing the animals' DNA.
Alignment and analysis of unique mutations was done using SeqMan 5.07 (DNASTAR Inc).
Editing of data and scoring of mutations were done by two independent groups of researchers.
Detection of mutations was done by analyzing the alignments, and genomic positions which were consistently (>60%, coverage of 5 or more reads) different were marked as mutated.
Mutation calling and filtering for the later set of mutations was done as described above.
Screening for EGFR (exons 18 21) mutations was done by PCR single-strand conformational PCR single-strand–SSconformational
The interpretation of resistance mutations was done through the Stanford HIV Db, Genotypic Resistance Interpretation Algorithm, version 6.2.0.
Analysis of sequence mutations was done to study the polymorphism of NPR1 in fifteen accessions of the Vitaceae family.
Alignments and analysis of mutations were done with MUSCLE in MEGA5.2 (Edgar 2004; Tamura et al. 2011).
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