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The fact that primary tumour and related metastasis could be different in terms of gene mutations is confirmed by K-Ras analysis in non-small-cell lung cancer (Kalikaki et al, 2008).
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Mutations were confirmed by sequencing.
The presence of the mutations was confirmed by DNA sequencing.
Successful introduction of artificial mutations was confirmed via both Sanger sequencing and the amplification refractory mutation system.
Blind validation of HI-unrelated patients revealed mutant alleles in 13 samples, and these mutations were confirmed by Sanger sequencing.
Specificity of mutations was confirmed by sequencing.
Mutations were confirmed by DNA sequencing.
The mutations were confirmed by sequencing.
The mutations were confirmed by PCR analysis.
All the mutations were confirmed by sequencing.
All constructs and mutations were confirmed by DNA sequencing.
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