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This discrepancy might be due to mutations introduced into the mutated 5′ss, which do not sufficiently compromise (miR163) or even favour (miR172) the binding of U1 snRNP.
Stewart et al. studied the transmission of mtDNA mutations introduced into the mtDNA mutator mice due to the expression of a proofreading deficient mtDNA polymerase [49].
In mutational cross-linking studies, these authors showed that double cysteine mutations introduced into TM1 and TM11 formed five different disulfide cross-links, but only if the transporter catalytically turned over via ATP hydrolysis.
(b) Missense mutations introduced into OsIRE1.
Point mutations introduced into Rv1625c to engineer a forskolin-binding pocket created a single heterodimeric catalytic centre, yet did not result in forskolin activation.
In contrast, all mutations introduced into putative SL4, SL8, and the stem of SL6 prevented replication, suggesting that the primary structure of these regions make up the core of the 3′ replication signal.
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Strain PA103exsAΩ is unable to express the T3SS due to a mutation introduced into a key transcriptional activator, ExsA (Table 1) [48] [48].
This observation shows that the point mutation introduced into clathrin heavy chain, impacts the behaviour of DT40 cells in a temperature-dependent manner.
The same mutation introduced into a different genetic background (hESC) results in a very similar electrophysiological phenotype.
A human GEFS+ mutation introduced into the orthologous mouse SCN1A gene suggests that a general outcome of deleterious SCN1A mutations is decreased activity of GABAergic interneurons.
In addition to the point mutation introduced into the ATP binding pocket, two Loxp sites were inserted into introns flanking exon 7 to 12 of the TrkA gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com