Sentence examples for mutations introduced by from inspiring English sources

Interestingly, the frequency of mutations introduced by gRNA complementary to the deletion mutant is significantly higher than with gRNA complementary to WT donor.

Point-mutations were confirmed by restriction digestion of plasmid DNA with Eco 72 I for mutations introduced by pair #1, and Eco 147 I for mutations introduced by pair #2, as well as by sequencing.

We speculated that such decline in cell growth was caused by the random mutations introduced by GREACE.

Using the HIV-1 env and pol genes as templates, four random libraries were generated representing different combinations of mutations introduced by up to 36 mutagenesis primers in a single assay.

Even without any positive selection pressure, the random process of mutations introduced by the virus polymerase in the expanding population of viruses will on average produce viruses that contain the required single, double, or triple mutations and even some quadruple mutants.

Assuming a Taq DNA Polymerase error rate about 1×10−4 base substitutions/bp/cycle, the total number of mutations introduced by our nested PCR protocol is around 8.24 base substitution per sequence (i.e. 1×10−4×758 bp×110 cycles (75 PCR cycles +35 Big Dye cycles)).

Therefore, increased susceptibility to disease may accompany a fitness-increasing mutation introduced by positive selection.

Characterization of precise genetic mouse models with the equivalent mutation introduced by knock-in techniques into Hdh, the mouse HD ortholog, indicates that molecular differences are evident even at the embryonic stem (ES) cell stage [ 9, 10].

We propose that M1dG → T mutations are introduced by hPol η and M1dG → A mutations are introduced by a combination of hPol ι and hPol κ.

The S to A mutations were introduced by PCR-based site-directed mutagenesis using primers carrying mutations for the respective S to A replacements.

Mutations were introduced by site-directed mutagenesis, using synthetic primer pairs encompassing the mutation site (Table 2).