This addition, one might say, is an adaptive mutation intended to maintain Darwinian theory against one of the many threats to its survival arising from its environment of inconvenient facts.
The introduction of p53 heterozygous mutation was intended to shorten the latency of tumor development in the RIZ1 knockout mice [7].
We suspect that Cys292 in the DECH-box motif might be needed, but since the protein did not tolerate the site-directed mutation we intended to construct, we were not able to test that hypothesis.
In order to gain further insight into the roles of these dimers, we have engineered a series of mutations in PhoB intended to perturb each of them selectively.
As mesalazine and TQ reduce the mutation rate at microsatellites in vitro, we intended to measure microsatellite mutations from intestinal epithelial cells and tumour tissue after microdissection.
To create N-terminal deletions, which can be successfully sorted to the outer surface, we intended to generate mutations starting at the +7 position.
The point mutation (S229P) in the hinge region, intended to stabilize covalent dimerization of the heavy chains, has been included in the sequence of other Pfizer mAb-calicheamicin conjugates, including the formerly licensed product gemtuzumab ozogamicin (Mylotarg®).
The point mutation (S229P) in the hinge region, intended to stabilize covalent dimerization of the heavy chains, has been included in the sequence of other monoclonal antibody-calicheamicin conjugates, including the formerly licensed product gemtuzumab ozogamicin (MYLOTARG™, Pfizer).
Although we intended to differentiate between standard and slow mutation rates, it was not intended to infer their exact values.
These data are intended to assist with the assessment of the likely pathogenicity of each missense mutation.
