We compared the deduced amino acid sequences of HKT1 among Arabidopsis, Thellungiella, and wheat to search for point mutations inducing higher salt tolerance in Thellungiella HKT1.
Each of the three cadherin mutations in P. gossypiella increased gut permeability to the cotton phytochemical gossypol, and costs were increased most by mutations inducing higher gut permeability (Williams 2009).
From PAGE analysis, we conclude that mutation induced high protein secretion that was evident from thick intense protein bands at equal loading volumes of extracellular proteins from each culture broth.
14, 15 This mutation induces high β-catenin protein levels and enhances TCF/LEF-dependent transcriptional activity.
Comparing the data from the patient and the unaffected father shows that the COL4A2 G702D mutation induces high levels of ER retention, ER stress and UPR activation in the patient but not the unaffected father.
Moreover, recovered recombinant viruses with the nsp1 β mutations by reverse genetics induced higher level gene expression of type I IFNs than that of wild type viruses.
Indeed, at least five cholesterol recognition/interaction amino acid consensus (CRAC 29 were identified in the ABCG2 transporter, but while mutation in Y413 and Y570 induced higher levels of ATPase activity that were not dependent of cholesterol presence, only one (Y413) was able to modulate ATPase activity in the presence of cholesterol30.
In contrast, homodimeric TALENs induced higher mortality during embryogenesis, which prevented us from detecting mutations.
While most frequent mutations that induce high levels of macrolide-resistance included an A-to-G transition at position 2063 or 2064 [ 11, 12], recent epidemiologic studies have suggested that distribution of MR- M. pneumoniae genotypes has been changing [ 4, 13].
Both G[8,5-Me]T and T[5-Me,8]G induced high frequencies of semitargeted or locally targeted mutations in addition to targeted mutations.
