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In contrast, many more mutations are effectively neutral in D. miranda, but the average effect of the mutations incorporated would be larger.
This increases the number of mutations incorporated in viral genomes over time and settles the ground for a relatively rapid genetic diversification [ 46].
These noncovalent interactions are influenced by the specific mutations incorporated into the set of Ht c-552 variants examined in this work.
The phenotype conferred by the UBP7 mutant does not reconstitute the full HWSSL tolerance displayed by R57 and supports the hypothesis that the high level of tolerance shown by R57 is a result of several mutations incorporated through GS.
The shape of the melting curves could easily be used to distinguish the mutation, targeted by each CADMA assay, from other mutations detected by the overlapping primer, as the resulting amplicons have different melting properties, due to the two additional mutations incorporated by the mutation specific primer.
The existence of multiple death pathways is an in-built strategy to protect the organism against abnormalities arising in a single or multiple pathways, making the occurrence of diseases like cancer relatively rare, considering the large number of cell divisions and mutations incorporated during the lifetime of a multicellular organism [ 1].
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Indeed, for SSG mutagenesis, because the number of ura3Δ isolates increased as the region of homology increased, we recovered many independent mutants containing a mutation incorporated 550 bp from URA3.
Once the mutant genes were constructed, they were sequenced in their entirety to ensure that the desired mutation was incorporated and no other mutations were generated.
Cysteine mutations were incorporated randomly into TIP47 by expression in Escherichia coli cells harboring specific misincorporator tRNAs.
In the two SSG mutageneses described above, mutations were incorporated 36 51 bp from the URA3 insertion site.
Therefore, the MnP1 D261G and I198F single and double mutations were incorporated, and the kinetic constants compared with those of native MnP1 and VP1 (Additional file 2: Table S6).
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