Sentence examples for mutations in practice from inspiring English sources

Although the global assembly paradigm has the potential to detect larger mutations, in practice it is less sensitive and more time consuming because a large proportion of reads used for assembly are not from candidate indels.

In theory, whole-genome sequencing (WGS) provides an ideal method for mapping mutations; in practice, however, given that a stock identified in an ethyl methanesulfonate (EMS) mutagenesis screen may reveal hundreds of coding-changing lesions, WGS approaches may require multiple alleles to unambiguously identify the lesion of interest (Blumenstiel et al. 2009; Osterloh et al. 2012).

However, the large gene sizes and lack of mutational hotspots make it difficult to survey for disease-causing mutations in clinical practice.

Up until now, DNA from archival tumour tissue is used to determine KRAS mutations in clinical practice.

Thus, even in Europe and the US where frequencies of EGFR mutations are low, incorporating testing for EGFR mutations in clinical practice may provide a huge benefit to some patients.

However, information about the frequencies and clinicopathological features of these mutations in clinical practice, including the relationship between mutation status and the efficacy of anti-EGFR therapy, especially among Asian populations, is still limited.

From database of two French pathology departments which detect KIT and PDGFRA mutations in routine practice (Ambroise Pare Hospital, Boulogne; Bergonie Institute, Bordeaux, France), we searched retrospectively for all consecutive patients with GIST and with either delWK or deletions including both residues Tyr568 and Tyr570 (delTyr).

Hence, screening for this mutation in clinical practice is not justified.

Objectives: The aim of this study was to investigate the clinical and virologic responses to treatment with NFV-containing regimens for up to 108 weeks and determine the timing and rate of emergence of primary NFV-resistance associated mutations in daily clinical practice.

Considering limited infrastructure demands and timescales for these techniques, we used the recently developed Snapshot assay to detect multigene mutations in routine clinical practice.

Mutations generated using a fluctuation assay can, in principle, arise anywhere in the genome; however, in practice these mutations very often arise in a handful of genes known to be important in whatever selective medium was used.