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Position 568 is under positive selection and is next to the polymorphic site R569W, mutations in position 569 have been shown to suppress posttranslational processing in MPO [ 42].
Mutations in position 77 were generated using the non-infections plasmid, p98CN009.8, containing the near full-length HIV genome [37].
Based on our data we conclude that a mutation at position E2-60 influenCHIKVHinfectivityvity for Ae. albopictus, regardless of the mutations in position E1-226, alsoalso modulates CHIKV infectivity for Ae. aegypti.
In stem I we found mutations in position 2.2 which in some instances were compensated for by mutations in position 1.2 in the same sequence, but in other instances were not.
Nevertheless, both works demonstrate maximum CG dinucleotides mutations in position 16 (around 30%) and in position 122 (about 26%).
In F9 exon 5, fix9 ExSpeU1 completely rescued the defective donor sites of the three synonymous mutations in position −2 (Fig. 6A, lanes 5 10).
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Thus the non-synonymous mutations in positions 30, 50 and 627 which were exclusively different between HTM and WT as well as those different from both WT and Nipponbare (81 and 152) were considered as potential causal mutations.
Finally, the Vλ VI sequences show mutations in positions 43 (FR2), 52 (CDR2), and in the FR4 region between 95ab 96.
Previously, a collection of small substrates possessing both single and multiple mutations in positions −1 to −4 was studied [49].
These alternative primers contain compensatory sequence variations to accommodate the frequently found mutations in positions 7847 and 7848 (Rev F 7830.1 and Rev F 7830.2).
Particularly, in the three positions where our data set had enough statistical power, we found evidence of associations with HLA alleles: two of them were already identified as potential CTL-escape mutations (Mutations in positions 30 and 83, Table 1) and mutation at position 84 had an OR of 0.34 and a p-value of 0.0716 for association with HLA-A02.
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