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For example, a mutational process could cause 1,000 mutations in one cancer genome while causing 20,000 in another.
Studies have indicated that on average 30% of breast cancers contain mutations in one of the p53 alleles [ 29].
Approximately 25% of human breast cancers contain missense mutations in one p53 allele, leading to inactivation of the mutated protein.
To clarify the effects of mutations on the tolerance phenotype, it is necessary to reconstruct selected mutations in one type of strains in various combinations.
First, two of the total 10 patients were excluded for low number of mutations in one of the trials.
We observed the simultaneous incorporation of up to 12 oligonucleotides with as many as 60 targeted mutations in one transformation.
PC is caused by mutations in one of four keratin genes; however, most patients carry K6a mutations.
This adduct induced >95% G→T mutations in one sequence context (5′-TGC) and approximately 95% G→A mutations in another context (5′-AGA).
E. coli ClpB (EClpB) harboring single mutations in one of the two ends of the MD coiled-coil, E432A and Y503D, are repressed and hyperactive mutants, respectively27,28.
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We observed somatic K-ras mutations in one-third of the lung carcinomas studied but no N-ras mutation.
Thirteen types of multi-mutations in one fragment were observed, among which 3 types were common combinations (≥5%).
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