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Most of the mutants carried mutations in marR.
Seven of the eight cyclohexane-tolerant mutants carried four different mutations in marR.
Mutations in marR can increase the expression levels of AcrAB and TolC proteins, which are components of the AcrAB-TolC efflux pump (Barbosa and Levy 2000).
To identify these mutations in organic solvent tolerant E. coli, eight cyclohexane-tolerant E. coli JA300 mutants were isolated and examined by DNA sequencing for mutations in marR, soxR, and acrR.
Mutations in marR or soxR were suggested to enhance the expression level of the AcrAB-TolC efflux pump (Okusu et al.1996 Aono 1998 Randall and Woodward 2002; Komp Lindgren et al.2003).2003
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Strain CH7 had a nonsense mutation in marR and an insertion of IS5 in acrR.
Among all mutants, strain CH7 carrying a nonsense mutation in marR (named marR109) and an insertion of IS5 in acrR, exhibited the highest organic solvent-tolerance levels.
Three of the seven mutations found in marR caused amino acid substitutions in MarR at the amino acid positions of L78, R94, and G116, and four of the seven mutations led to a translation termination codon at the position of E109 (mutationmutation).
In this study, two mutants (CH1 and CH7) carried mutations in both marR and acrR.
The other two mutants (CH1 and CH7) each carried mutations in both marR and acrR.
PCR followed by sequencing and in silico analysis revealed the contribution of mutations in acrR, marR and gyrA to CIPR development.
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