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All mutations in line 125 were heterozygous.
Overall a significant genomic drift was seen favoring GC → AT point mutations in line with the chemical mutagenesis strategy used for generation of the cell line.
Our cohort of newly diagnosed HIV-1 infected patients confirms a prevalence of drug resistance mutations in line with other reports throughout Europe.
In particular two sets of genes, named the NMDA receptor and Arc complexes, showed increased association with SCZ mutations, in line with earlier studies that also pointed toward synaptic genes implicated in SCZ and BD.
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The present study revealed normalization inaccuracy or sampling bias by detecting homozygous mutations in lines that should have yielded only heterozygous mutations.
The missense mutation for line 17D was in a highly conserved region of the protein sequence, while the missense mutations in lines 615 and 743 were in less conserved regions[ 24].
I found evidence for three fixed novel mutations in total: one coding mutation in line 16EE6 (a missense mutation in nhl-2 ), and one noncoding mutation each in lines 18EE1 and 18EE2 (Table 1).
Four more silent mutations were found in Ara h 2.01, one of which is identical to the silent mutation in line 37-4.
The mutation in Line 687 (Megf8 L1775P ) is predicted to encode a single L>P amino acid substitution (L1775P) in the fourth Kelch domain of Megf8.
The mutation in line P5A3 (proline in wild type to leucine in mutant) is predicted by PSSM and SIFT scores to be less damaging to the protein function than the mutation in P7H6 (glycine in wild type to serine in mutant, Figure 4), both lines displayed brown midribs in leaves and light brown color in stem-cross sections.
Splicing mutations in cell line U2OS predicted by Shannon pipeline.
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CEO of Professional Science Editing for Scientists @ prosciediting.com