Sentence examples for mutations flanked by from inspiring English sources

This study showcased the difficulty of obtaining stable mutants in most Cyanothece strains and how to improve transformation efficiency by introducing linear, single-stranded, oligonucleotides carrying the desired mutations flanked by fragments homologous to a chromosomal insertion site.

Characterized by an extremely high sensitivity detection of mutations, the snapback primer system can identify almost all the mutations flanked by the snapback probe during the melting curve analysis.

To determine the activity of LP-incorporated ZFNs, we established a reporter system in HEK293 cells (HEK293-eGFPmut reporter cells) based on the genomic insertion of a Sleeping Beauty DNA transposon (carried by the plasmid pT2/CMV-egfp-mut.SV40-neo) harboring an egfp cassette gene from which expression was inhibited by stop- and frameshift-mutations flanked by the two ZFN recognition sequences.

Briefly, complementary primers, containing the desired mutation flanked by 15 nucleotides corresponding to the APN cDNA sequence, were used to amplify the complete cDNA-containing pGem-He-Juel vector.

HDR can be exploited by the introduction of an exogenous repair template that harbors a desired mutation flanked by homology arms, thereby greatly improving upon the efficiency of traditional homologous recombination, in which the initiation of the process (generation of a DSB) must occur spontaneously.

Because most of the target mutations were flanked by genetic markers that are already identified to the level of the gene sequence, the amount of the genome that require examination for each strain ranged from a low of 74 kilobases (kb) in strain FGSC 305 to over 4 megabases (Mb) in strain FGSC 3562 (Table 1).

The AS160T649A knockin mouse, in which the tenth exon harbouring the T649A mutation is flanked by loxP-Cre excision sites, was previously generated in our laboratory [ 12].

We previously reported the generation of an AS160T649A knockin mouse in which the tenth exon harbouring a T649A mutation is flanked by loxP-Cre excision sites [ 12].

Decisions on whether or not a mutation was flanked by two markers were based on whether recombinations with the markers occurred independently.

After integration of the selection marker, a second plasmid carrying a mutation cassette, again flanked by I-SceI sites, is introduced.

In contrast to the Arctic mutation, the Swedish and London mutations flank the A β domain.