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The number of nonsynonymous mutations expected for different clone population sizes is shown in Additional File 4 and is described more fully later.
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These 17 mutations were G/C-to-A/T mutations, as expected for EMS [ 17].
For multiple rare mutations expected to affect phenotypic traits of interest, grouping variants from the same genes, pathways, and segmental conserved regions has provided promising results (Bodmer and Bonilla 2008; Cohen et al. 2004; Nejentsev et al. 2009).
Thus, a relatively higher proportion of silent mutations are expected for "functional genes" underlying natural selection.
For example, there is an unusual mutagenesis pattern in the OMM substrate for the single MLH1 and MSH2 knockdowns compared to the double knockdown that makes me concerned based on the high mutation rate expected for single knockouts of these critical MMR genes.
Under rare conditions for I-Bmol, it is possible that up to 20 mutations could be expected for this 266-site protein, making it likely that interaction effects are non-negligible factors of selection.
The observed decrease in mutation ratios was expected for all time-windows as the high ratios of external branches also contributed to the ratios of the older clades (Figure 5).
The resulting composition in the act1 fadoubleble mutant line grown at 17°C indicates the profile expected for mutations at each of these steps.
A variation in the m-value of this magnitude is not expected for mutations that have little effect on the secondary structure (Figure S1) and retain the dimeric form.
Such a difference in the rate of evolution is not expected for mutations with female-limited fitness effects and is most likely to occur for mutations with male-limited fitness effects.
We also checked whether the haplotype variation in our sample is consistent with the characteristic star phylogeny expected for mutations that arose on the background of the sweeping founder haplotype.
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