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Cellular analysis and manipulation of tracheal cell number using cell-cycle mutations demonstrated that tube size is not dictated by the specific number or shape of the tracheal cells that constitute it.
RNA interference in tissue culture cells, live analysis of chromosome segregation after antibody injection into early embryos, and observations of flies with CENP-A null mutations demonstrated that CENP-A is required for all chromosome movements during mitosis and for normal cell cycle progression.
Analysis of mucA mutations demonstrated that 75% corresponded to the mucA22 allele, with the remaining 25% being represented by C-to-T transitions (Table 1 and Figure 2C).
Controls spiked with each of these two deletion mutations demonstrated that one mutant molecule can be detected in 1.7 3.3 µg gDNA (0.5 1×106 genomes) and that the samples lacked inhibitors at the concentrations of genome utilized.
Analyzing gene expression and the role of individual mutations demonstrated that we had selected for more than multicellularity.
However, analyses of different mutations demonstrated that each mutation has a unique effect on the biochemical and biophysical properties of the LMM [ 4].
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Only one case presented a BRAF mutation, demonstrating that is a rare alteration in EAL.
Screening of mutations demonstrates that the C-domain (residues 29 40 (42)), the median domain (residues 17 22) and the N-domain (1 16) are all crucial for interaction.
Surprisingly, all six residues correspond to sequence positions associated with drug resistance mutations, demonstrating that the very residues that are responsible for native substrate specificity in HIV-1 protease are altered during its evolution to drug resistance, suggesting that drug resistance and substrate selectivity may share common mechanisms.
In fact, in the past decade, forward genetics has uncovered mutations demonstrating that certain genes exist only in mammals, that specific mechanisms function only in mammals, and that particular biological processes may exist only in mammals; hence screens focused on these processes have identified unsuspected genes.
Examination of the published KLF6 mutations demonstrates that indeed a number of the cancer-defined mutations occur in the NLS and NES domains (Figure 8).
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