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The name Mutator originates from the ability of active copies of this element to induce mutations corresponding to diverse recombination events, which were first described in plants (Bennetzen 1984; Jiang et al. 2011) and later in other organisms (Amyotte et al. 2012).
Mutations corresponding to cof1-5 and cof1-22 [26] were introduced to Cof1 expression plasmid by site-directed mutagenesis.
To mutate internal Age I, Xho I, and Bgl II sites of the pyrimethamine resistant Toxoplasma gondii dihydrofolate reductase-thymidylate synthase gene (TgDHFR-TS, 1.846 kb), the ORF was amplified in three fragments (TgDTA: F1/R1; TgDTB: F2/R2; F3/R3) using Phusion DNA polymerase and primers containing one base silent mutations corresponding to the restriction enzyme sites.
Figure 3 shows the minimum effective size Nemin required for selection to overcome genetic drift for mutations corresponding to a range of altered means (Figure 3A) and variances (Figure 3B) of the cumulative risk Fbo(x) estimated for BRCA1 alleles in the case of the population of Quebec (red line in Figure 3A and 3C).
All these mis-sense mutations corresponding to the CAD reading-frame are illustrated in Figure 3.
Then, the mutations corresponding to haplotype lineages were paired with their normal tissue sample.
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The model is based on a point mutation corresponding to the p53 mutated hotspot p53.R273H in the human Li Fraumeni cancer syndrome.
However, as reported by Reeve et al. (Reeve et al., 2008), dFmr1 E68K mutation (corresponding to FMRP E66K) showed a similar dorsal axonal elaborations of ventral lateral neurons to dFmr1 homozygous null mutants (Reeve et al., 2008), while by homology, FMRP E-66 is an important residue that is involved in forming salt bridge that serve to stabilize Agenet domains.
The '@' symbol in the inset indicates the C-T transition mutation corresponding to the H275Y mutation.
A C3d construct, engineered to include a free exposed thiol group, was generated by reversing the initial Cys17Ala mutation (corresponding to Cys1010 of full length C3).
In addition, when FoxP2 was knocked out [31] or a FoxP2 mutation corresponding to the human language disorder was knocked in [32], the number of isolation calls decreased.
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