Of these, most contained single mutations: eight had either H3 A7V or H3 A7T mutations, seven had an H3 T6I mutation, and one bore an H3 G33V mutation.
Sequence analysis of PCR products derived from four F1 animals out of the nine mutant F1 animals showed that two (B-1 F1 and B-2 F1) contained single mutations, and a third animal (A-1 F1) contained two mutations.
Four mutants, named IS4VHi, IS4VHii, IS4VHiii and IS4VHiv, contained single mutations of arginine residues at positions 96, 97, 100 and 100 g, respectively.
Sequence of the 10 mutant plasmids indicated that each plasmid contained single or multiple mutations in the rep gene or the flanking regions.
Control siRNA contained single nucleotide mutations within the gene-specific insert.
These first-step mutants contained single, non-synonymous mutations to gyrA and no other mutations (e.g. in efflux pumps) linked to ciprofloxacin resistance (Supplementary Data 1).
Of the fur genes from 20 transformants sequenced, ten contained single substitutions, six of which affected just one position in Fur protein (position 41); the other four transformants contained single mutations affecting protein positions 40, 41, 57 and 95.
However, the variants were shorter than expected, and contained single-nucleotide mutations, inserted and deleted exons, and larger deletions that led to the formation of new exon exon junctions between missing multi-exon regions.
Sixty-one of the 79 predicted pseudogenes in HL096PA1 contain single inactivating mutations, indicating that the majority of these mutations were recently acquired [ 34].
A large number of other single gene databases exists like the L1CAM mutation database from the university of Groningen (52) containing single gene somatic mutations.
