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Results of this proof-of-principle study on clinical samples indicate clinical utility of multiplex ddPCR for screening for multiple EGFR mutations concurrently with an ultra-rare pretreatment mutation (T790M).
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We identified one missense and one nonsense mutation in ASH1L (concurrently with SETD2 mutation) and SETD3 (1 missense mutation concurrently with SETD2 mutation).
Future studies will help further clarify whether LKB1 mutations occur concurrently with other genomic alterations in NSCLC and the impact of this on patient outcome.
10.7554/eLife.03297.010 Figure 4. DNA damage caused by Nde1 mutation occurs concurrently with DNA replication.
In our study, we were not able to detect a significant survival difference for patients whose tumours contained LKB1 mutations alone or concurrently with KRAS mutations (data not shown) likely because of the limited number of tumour specimens.
During this process numerous genetic mutations are lost from the population while mutations which occur concurrently with drug resistance causing mutations (or 'hitchhiking SNPs') remain [ 14].
Previous reports have suggested that in NSCLC cell lines, LKB1 mutations often occur concurrently with KRAS or BRAF mutations (Sanchez-Cespedes et al, 2002; Carretero et al, 2004).
The rtL180M mutation usually occurs concurrently with the rtM204V mutation and serves as a compensatory mutation [ 11].
None of the patients with t(15 17), inv(16), t(7 11) or 11q23 translocations showed TP53 mutation, but one patient with t(8 21) harbored this mutation concurrently.
Then, the emergence of P. brasiliensis RtPc1-specific lineages due to the accumulation of mutations can occur concurrently with the maintenance of copies of RtPc1 similar to those present in the common ancestor of P. lutzii and P. brasiliensis [ 37].
Sixty-nine (75.0%) of them had at least one Class II mutation concurrently.
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