Sentence examples for mutations changing the from inspiring English sources
Mutations changing the identified and candidate phosphorylation target sites were introduced by site directed mutagenesis into a functional, untagged mini-BicD gene, that is expressed from its native promoter and is also used as the wild type control gene (BicDwt; see Materials and Methods section for details).
First, only point mutations changing the allele type at a certain gene locus are being considered in these models.
At each generation we apply crossovers and mutations, changing the allocation of multi-membership genes to their member pathways.
Consistent with the role of GluCl as the main target of AVMs, IVM-resistant strains of invertebrates can show mutations changing the expression levels or the peptide sequence of channel subunits [ 23, 24].
In theory, silent mutations including mutations in noncoding regions and synonymous mutations in coding regions have less severe phenotypic effects than non-synonymous mutations, changing the amino-acid composition.
To generate mutants of CXCR4 differing in N-glycosylation we have introduced point mutations changing the coding sequence for asparagine, threonine or serine into the coding sequence for glutamine and alanine (Fig. 1b).
fkh1 contains an ethyl methanesulfonate (EMS -induced point mutation changing thEMS -inducedW254, a residue in the forkhead DNA-binding domain, into a stopointon, while the X-ray-induced allele fkh6 carries an 11 bp deletion which produces a framutationafter the changingr residue E7 [15].
The oligos added a SmaI site 357 bp upstream of the rad50 translational start site and added the K81I mutation (changing the codon from AAA to ATT).
On the contrary, if it was paired, a neutral base pair mutation (changing the selected nucleotide and its pair) was applied.
Such a pattern implies that a mutation changing the encoded amino acid sequence is much less likely to be different between two species than one which is silent.
Sequencing of the apoc2 mutant revealed a one-nucleotide replacement and ten-nucleotide deletion, resulting in a frame shift mutation changing the coding sequence of the Lpl-binding domain of the Apoc2 protein (Fig. 1E).
