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We have developed a web server for predicting the protein stability changes upon single mutations by using fuzzy query mechanism and it is available at http://bioinformatics.myweb.hinet.net/fqstab.htm.net/fqstab.htm
Expression scores in Fig. 2 were calculated as the geometric mean of TPM values of genes in Supplementary Data 3. Genomic DNA extracted from Pat208 samples was used to validate the WES c.(37 39 ctcfs, p.Leu13fs; and c.(40 45 tctcttfs, p.Ser14fs mutations by using targeted Sanger sequencing.
Finally, colony PCR reaction was performed to verify the success of the mutations by using oligonucleotide primers 425-TT-BamHI-FW/426-TT-EcoRV-RV as referred in Table 2.
We study the outcome of immune escape mutations, by using two-strain dynamical models applied to the resident and the mutant antigenic clusters defined here above.
The isolates were also examined for gyrA and parC mutations by using primers and methods described (7 ).
2- Denaturing high-pressure liquid chromatography (dHPLC) was used to screen NRAS mutations by using a Transgenomic Wave machine (Transgenomic, NE, UK).
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To assess macular photoreceptor abnormalities in eyes with fundus albipunctatus with RDH5 mutation by using adaptive optics scanning laser ophthalmoscopy (AO-SLO).
METHODS: We screened 653 microdissected, newly diagnosed invasive breast tumors from patients in the Carolina Breast Cancer Study, a population-based case-control study of breast cancer in African American and white women in North Carolina, for the presence of the ER-alpha A908G mutation by using single-strand conformational polymorphism (SSCP) analysis and 33P-cycle sequencing.
Progression-free survival rate for tumors positive for the germline mutation was compared with those negative for the mutation by using the Kaplan-Meier method.
Enrichment for the mutation by using MboII restriction digestion allowed the detection of mutant present at 1% or less.
18 discovered a resistant ALK mutation by using pleural effusion cell DNA obtained from an EML4-ALK lung cancer patient who acquired crizotinib resistance.
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