Sentence examples for mutations by use from inspiring English sources
Exons 4 to 8 were screened for mutations by use of denaturing gradient gel electrophoresis in accordance with the method described by Hamelin et al (1993) for exons 5, 7, and 8, and the method of Gulberg et al (1997) for exons 4 and 6.
Expression scores in Fig. 2 were calculated as the geometric mean of TPM values of genes in Supplementary Data 3. Genomic DNA extracted from Pat208 samples was used to validate the WES c.(37 39 ctcfs, p.Leu13fs; and c.(40 45 tctcttfs, p.Ser14fs mutations by using targeted Sanger sequencing.
We have developed a web server for predicting the protein stability changes upon single mutations by using fuzzy query mechanism and it is available at http://bioinformatics.myweb.hinet.net/fqstab.htm.net/fqstab.htm
Finally, colony PCR reaction was performed to verify the success of the mutations by using oligonucleotide primers 425-TT-BamHI-FW/426-TT-EcoRV-RV as referred in Table 2.
We study the outcome of immune escape mutations, by using two-strain dynamical models applied to the resident and the mutant antigenic clusters defined here above.
The isolates were also examined for gyrA and parC mutations by using primers and methods described (7 ).
2- Denaturing high-pressure liquid chromatography (dHPLC) was used to screen NRAS mutations by using a Transgenomic Wave machine (Transgenomic, NE, UK).
These samples had been previously evaluated for EGFR mutations by using routine diagnostic techniques including direct sequencing, fragment analysis, real-time PCR-allelic discrimination, and the EGFR Therascreen RGQ kit.
Another solution could be to re-order the 10% defective primers and re-clone the defective clones or to correct the mutations by using mutagenesis kits.
The objective of our investigation was to provide a detailed description of the behavioral phenotype associated with GRIN2B mutations by using clinical assessment as well as standardized questionnaires.
We simulated random mutations by using a compositional substitution matrix based on the nucleotide frequencies of the original (wild type) genome [ 47].
