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Furthermore, to validate the proposed assay for potential applications in clinical diagnostics, we examined paraffin-embedded tissue samples from 10 NSCLC patients for the presence of EGFR mutations by performing EGFR-DMP and direct sequencing.
In addition, EMU can increase the precision of the predicted mutations by performing sequential checking.
Under this approximation, the distribution of net ΔΔ G values for multiple mutations can be computed from the distribution of ΔΔ G values for single mutations by performing convolutions of the single-mutation ΔΔ G distribution [ 3], meaning that W m for arbitrary m can be computed solely from the distribution of ΔΔ G values for single mutations.
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Finally, we measured the sensitivity of our mutation detection assay by performing a dilution of mutation template into increasing amounts of wild-type genomic DNA lacking the mutation.
All mutations were confirmed by performing two independent PCR amplifications.
Identified mutations were reconfirmed by performing an independent PCR reaction from a fresh aliquot of stock DNA.
The plasmids carrying sopB mutations were created by performing site-directed mutagenesis using the Pfu Turbo DNA polymerase (Stratagene) as per manufacturer's instructions.
To map the mutation, we began by performing complementation tests using chromosome 2 deficiency stocks.
We directly test the stop codon mutation (SCM) mechanism by performing a detailed case study of Escherichia coli overlapping sequences.
The function of BY-kinases has been mainly studied by performing mutations in their catalytic site.
We sought to evaluate the role of germline BRCA1 mutations in PrCa predisposition by performing a candidate gene study in a large UK population sample set.
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