Sentence examples for mutations by denaturing from inspiring English sources

The Fas gene was screened for mutations by denaturing gradient gel electrophoresis of the extracted DNA.

Exons 2 11 were screened for mutations by denaturing high-performance liquid chromatography (DHPLC) using the Wave® (Transgenomic, Inc., Omaha, USA).

DNA from the 38 CRC patients were screened for MSH6 mutations by denaturing gradient gel electrophoresis (DGGE) using primers and conditions described previously (Wu et al, 1999).

The MLH1 and MSH2 genes were pre-screened for mutations by denaturing gradient gel electrophoresis (DGGE) as described in [12] and PCR fragments showing an aberrant DGGE profile were subsequently sequenced to determine the underlying mutation.

The complete ATM open reading frame was analysed; each exon (exons 4 to 65) and corresponding splice sites were screened for germline mutations by denaturing gradient gel electrophoresis, identifying 80to90%0% of all ATM mutations and polymorphisms [ 29].

DNA was first extracted and exons 4 to 8 were screened for mutations by denaturing gradient gel electrophoresis (DGGE) in accordance with the method described by Hamelin et al (1993) for exons 5, 7 and 8 and the method of Gulberg et al (1997) and colleagues for exons 4 and 6.

TP53 mutations were assessed by denaturing gradient gel electrophoresis analysis and DNA sequencing.

PTEN point mutations were identified by denaturing gradient gel electrophoresis (DGGE) screening followed by sequencing of the variants on an ABI DNA sequencing machine [ 11].

To determine whether these mutations occurred in response to UV, we exposed engineered human skin (EHS) to UVA, UVB, or a mixture that resembled sunlight, and then detected mutations by both denaturing high-performance liquid chromatography and DNA sequencing.

First, all coding regions and flanking intronic regions were screened for mutations or polymorphic variants by denaturing high-performance liquid chromatography (DHPLC), a mutation-screening technique that resolves homoduplexes and heteroduplexes of DNA formed by variant and wild-type sequences by high-performance liquid chromatography.

This may have been due to the application of insensitive genetic screening techniques given that direct sequencing revealed many CCM1 gene mutations that had been missed by denaturing high-performance liquid chromatography or SSCP in the past.