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Previous studies suggested that CRISPR/Cas9 system would probably induce off-target mutations because the binding to genome could tolerate sequence mismatches distal from the PAM at the 5′ end of sgRNAs.
However, it is difficult to infer more information on recent H. pylori evolution from the H. acinonychis mutations because the sequences have been changed to a great extent.
It was not possible to combine the designed AhR and USF motif mutations because the core motifs overlap extensively in the nucleotide sequence (Fig. 4A).
Based on the sizes of InDels, 28.6% of InDels were frame-shift mutations, because the numbers of nucleotides in the InDels were indivisible by three.
They used the term "functional virginity mutations" to describe these mutations because the phenotypic result is females that no longer fertilize their eggs.
In these subjects the first exercise test alone could not distinguish idiopathic adrenergic VAs from those linked to RyR2 mutations because the latter were identified by family screening.
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However, it is somewhat premature to compare the effects of different murine mutations because, to date, the mutations have not been systematically evaluated on a uniform genetic context.
However, there was no loss of enzymatic activity despite the two mutations, because when the purified proteins were tested for catalytic activity 188 U mg−1 was measured for MFECP and 210 U mg−1 for MFEdmCP.
Massively parallel ('next generation') resequencing is potentially a powerful tool with which to study heteroplasmic mitochondrial DNA (mtDNA) mutations because of the possibility of the high breadth and depth of coverage.
The PM localization of PIN3 in root hair cells was disrupted and internalized by the phosphorylation-defective mutations because of the defect in PM targeting.
These suppressors were allele-specific and gene-unspecific and were thought to act on nonsense mutations because of the lack of any residual function in the gene products of the suppressible mutant strains.
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