The next most common position mutated in FIPV is P2; >30% of the FIPVs analyzed bore mutations at this position.
The MACSIMS annotation provides several descriptions of conservation, such as the conservation score of the substituted position, the percentage of mutated residues at the same position and the number of known mutations at this position.
The Y641 residue is the most frequently mutated residue, with up to 22% of germinal centre B-cell DLBCL and follicular lymphoma harbouring mutations at this site.
To further determine whether other substitutions at His547 show differential effects on the basal DA transport, mutations at this residue (histidine to proline, arginine, and aspartate; H547P, H547R and H547D) were generated by site-directed mutagenesis.
The fact that all possible mutations at this particular site result in increased activity suggests that this region may function to constrain the G protein coupling of the receptor, a constraint which is normally relieved by agonist occupancy.
The results showed that all of the male sterile plants had homozygous mutations at this locus, whereas the fertile plants were homozygous wild type or heterozygous genotype (Fig. 4b and Table 1), indicating this mutation was co-segregated with the male sterile phenotype of oslap6, also suggesting that LOC_Os10g34360 plays an essential role in rice male reproductive development.
However, its structural integrity is still important for function as extensive but not limited mutations at this interface diminished the activity of the receptor.
This potential CASPASE-7 cleavage site needs to be confirmed in future experiments using recombinant OCT4 proteins bearing specific point mutations at this recognition sequence.
For example, in FlhB, mutations at this site inhibit the export of "late" flagellar proteins, while normal levels of early substrates, including hook protein FlgE, are secreted [27].
It has been previously shown that in other RAS family members, mutations at this site cause loss of GTPase activity with constitutive activation [3].
Mutations at this locus in BXO8 (PP and RS, unpublished), in Xcc8 [18], and in Xoc [83] are associated with reductions in virulence.
