Sentence examples for mutations at specific from inspiring English sources
There are also multiple examples of profiling the steps in evolving new metabolic capabilities in the 1984 Mortlock "Microorganisms as model systems for studying evolution" volume that follow the 'target-size' paradigm of a regulatory mutation knocking out a repressor first to achieve overexpression, followed by rarer refinement mutations at specific sites in an enzyme that change its specificity.
We show that functional expression of GLUT5 in yeast requires mutations at specific positions of the transporter sequence.
They were able to identify mutations at specific points, or loci, on three genes linked to fur length, curliness and growth pattern (bushy eyebrows, beards and other features that dog breeders refer to as furnishings).
Overall, our results demonstrate that targeted genome-editing methods are efficient in inducing mutations at specific loci in embryos of a non-traditional model species, without detectable off-target effects in paralogous genes.
This study examined the effects on the VPg uridylylation reaction of the RNA template sequence, the origin of VPg (coxsackievirus or poliovirus), the origin of 3D polymerase (coxsackievirus or poliovirus), the presence and origin of interacting protein 3CD, and the introduction of mutations at specific regions in the poliovirus 3D polymerase.
The relative frequencies of mutations at specific sites.
Plasmids encoding Rpb1 with a K to R mutation at specific sites were created using plasmid pJS670 as template through site-directed mutagenesis (QuickChange II mutagenesis kit, Stratagene).
In some taxa, it has been shown that the rate of mutation at specific loci is strongly correlated with GC content [ 26- 31].
Since the cellular level of p21 is reduced following its mutation at specific amino-acid sites that prevent p21 binding to PCNA, it has been proposed that PCNA protects p21 from proteosomal degradation (Cayrol and Ducommun, 1998).
This can also reveal the spatial dynamics of mutations at any specific positions.
MUPAC could efficiently introduce various mutations at multiple specific sites within a gene.
