Previous theoretical analyses did not identify the stabilization caused by the mutations at positions 1, 46, and 48.
The results demonstrated that mutations at positions 166, 312, 539 had effects both on the enzyme properties and catalysis of TsPOx.
Little change in folding rates occurred for the Ala/Gly pair mutations at positions in helix I and the C-terminal regions of helix II and IV.
The assay based on reverse hybridization principle simultaneously detects 13 different mutations affecting 6 independent codons, including the most prevalent mutations at positions 531 and 526.
(D) Overexpression of the human DSS1 gene with substitution mutations at positions W27, W39, W43, and F52 in HEK293F cells completely abrogated the UVB-induced DSS1-protein adduct formation.
We validate our approach by introducing a promiscuous esterase activity in E. coli thioredoxin on the basis of mutations at positions close to the native-active-site disulfide-bridge.
The reactivity across all clade specific mAbs centred around a constellation of mutations at positions 140, 145, 171 and 172, all of which are proximal to the receptor binding site on the membrane distal globular head of the HA.
After 5 rounds of panning, the mutations at positions 97 and 100a were identified.
Clonal analysis suggested that the mutations at positions 143 and 155 were exclusive and were selected independently.
For BRAF and KRAS, activating mutations at positions known to frequently carry alterations are reported [11], [58].
One such HIV-1 resistance pathway (Figure 1) is characterized by the key mutations at positions 41 and 215 of the RT [31], [33].
