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Some of the mutants contained multiple mutations, as shown in Fig. 1.
This system proved that the sensor is selective towards DNA and can be utilized for detecting mismatches in BRCA 1 gene mutations as shown in Fig. 3 (Rasheed et al. 2014).
Referring to the study performed by Liang et al., there was a low efficiency of HDR of the endogenous β-globin gene (HBB), production of mosaic embryos, and formation of off-target mutations as shown through whole-exome sequencing and T7E1 assays (Liang et al., 2015).
It could be caused by ProtFP (Feature)'s abilities to pick up point mutations (as shown in the NNRTI section), however containing too little information to accurately describe the bioactivity space (as shown by the bad performance of ProtFP (Feature) alone).
Sequencing analysis revealed that each mutant contains several mutations as shown in Fig. 1-D.
The analysis of the HA and NA glycoproteins revealed a large number of subtype-specific adaptive mutations, as shown in Figure 5 (details are given in Tables S1, S2, S3, S4, S5 in the Supplementary Materials S1).
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In some cases, they can even produce complex mutations (e.g. tandem base pair substitutions and clusters of multiple, closely spaced mutations) as was shown recently for Pol ζ (Stone, Lujan, Kunkel 2012).
In the text the authors refer to the clustered mutations as showing a "strong tendency towards strand polarity".
1) In the text the authors refer to the clustered mutations as showing a "strong tendency towards strand polarity".
In order to characterize a pair of subclonal mutations as showing subclonal evolution, we needed to find at least one example of a read pair reporting both variant alleles simultaneously AND one read pair reporting one variant allele but the wild-type allele for the other locus.
On the other hand, GA repeatedly generates solutions for a large population by applying the operators: (1) selection, (2) crossover and (3) mutation as shown in Fig. 5.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com