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The GFP used in this study possesses three-point mutations as compared to the Aequorea wild-type protein, namely S2A, F64L, S65T.
In addition, peptides derived from variable cleavage sites are generally more sensitive to the mutations as compared to those derived from conserved sites, supporting the co-evaluation mechanism of HIV-1 PR and its substrates under drug suppression.
That means that about one in 40 Ashkenazi Jews has one of these mutations as compared to one in 400 people having a BRCA1 and one in 800 having a BRCA2 abnormality in the general population.
This would suggest that XMRV has a considerably lower potential for developing drug-resistant mutations, as compared to HIV-1.
Organisms that reproduce through self-fertilization are thought to be at a lower risk of accumulating mutations as compared to outcrossing or asexual organisms because selfing promotes the expression of recessive alleles, which in turn makes these alleles more visible to natural selection, allowing them to become "purged" from the population [15] [17].
Conversely, the VH genes from the CD19+CD27+IgD− cells from patients, which would be expected to have a higher number of mutations, had a significantly reduced number of mutations as compared to the controls (15 vs 25 mutations per gene respectively, P<0.001; 4.8% vs. 8.3% bp sequenced).
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Moreover, gene expression, polyamine levels and enzymatic activities were increased in colorectal carcinoma samples expressing K-ras mutation as compared to non mutated K-ras samples.
PPARγ expression, as well as ODC and SSAT mRNA levels were significantly higher (p < 0.05; Mann Whitney test) in colorectal carcinoma samples expressing K-ras mutation as compared to non mutated K-ras samples.
Second trimester intrauterine fetal death occurred more frequently in cases with the factor V Leiden mutation as compared with the control group (8/64 versus 0/92).
The c-myc T58A gene bears a single mutation as compared to wild type c-myc and has never been used before to immortalize cells.
The occurrence of ASCA was higher by 25% in Crohn's disease patients carrying at least one NOD2/CARD15 mutation as compared to the non-carrier ones [46], [47], [48]; however, the ASCA prevalence was also high in this latter group.
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