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MtDNA mutations are randomly segregated to the forming oocytes during postfertilization of the female fetus.
There, mutations are randomly divided into training and test sets (or into cross-validation folds).
The positions of mutations are randomly determined, and haplotypes are written to a file for input into GemReads.
Therefore, if mutations are randomly added to an organism's genome (as opposed to incorporated by natural selection), then fitness tends to decline with mutation number.
(C ) Observed distribution of IMDs in the AID* dataset compared to a simulation assuming mutations are randomly scattered throughout the genome.
In the TILLING approach (Targeting Induced Local Lesions IN Genomes), point mutations are randomly created throughout the genome by treating seeds with a mutagen (e.g. ethyl-methanesulfonate (EMS)) [ 285- 287].
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This would not be the case if low level mutations were randomly generated false calls.
New mutations were randomly assigned to the individuals and then to random nonsegregating sites.
By contrast, the recessive mutations were randomly distributed throughout p53 and did not reside in the DNA binding domain.
As in SIM I, 100 sets of 30 driver genes were randomly selected from the Cancer Gene Census and non-silent mutations were randomly added at three levels.
Mutations were randomly introduced by error-prone PCR, and mutants with increased antibiotic resistance were selected using cefotaxime (CTX; Sigma, St . Louis MO, USA) and ceftazidime (CAZ; Sigma).
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