Exact(4)
Results from the experimental validations of above predictions of possible compensating mutations are provided in Table 4.
Detailed results from the GeneSV analysis of these mutations are provided in Supplemental File 2. GeneSV can be used for various purposes aimed at characterizing sequence variability.
The most obvious cases of canonical compensatory mutations are provided by alterations affecting the secondary helical structures of tRNA and rRNA molecules, whose effects are counterbalanced by changes restoring base pairing [ 14, 15].
Detailed results from the GeneSV analysis of discussed above 32 single codon mutations are provided in the supplemental data – Supplemental File 1. Two of the selected above single mutation positions K279 and R437 were also proposed for testing of possible compensating mutations.
Similar(56)
ago2 was provided by R. Carthew, and all other mutations were provided by the Bloomington Drosophila Stock Center.
A multiple sequence alignment highlighting the postions of disease-causing mutations is provided as additional information [Additional file 1].
A detailed view of the intra-tumoral prevalence of known GBM driver mutations is provided in Supplementary Figure S10.
A very telling example of compensatory mutations is provided by the mutations K6N+F11V+P20L (i.e. mutations 1, 3 and 4 placed in the context of the consensus sequence).
Details of each mutation are provided in Supplementary Table S1.
However, no clinical data or electrophysiological properties of this mutation were provided.
Flies with the Shmns mutation were provided by Dr. Chiara Cirelli (University of Wisconsin) and outcrossed six times into an Iso31 background.
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