Sentence examples for mutations and used from inspiring English sources

pSMT3 + CBM was extracted from single colonies that could grow in presence of kanamycin 50 μg/mL, sequenced to be sure there were no unwanted mutations and used to transform E. coli Rosetta (DE3) strain.

The PCR primers were designed to contain mutations and used to amplify DNA fragments.

We thus performed ethyl methanesulfonate (EMS) mutagenesis, isolated mutations, and used them to demonstrate transmission through males and Mendelian segregation in progeny hermaphrodites.

In this study we generated a bank of DNA, tissues, and sperm from 4,000 male progeny of ENU-mutagenized C57BL/6J mice, employed TGCE as a high-throughput and cost-effective gene-driven approach for identifying mutations, and used ICSI to recover live mice from cryopreserved sperm of males carrying specific mutations.

This data classifies the effects of the missense mutations and uses a universal value so that unfolding propensities from different protein structures may be compared.

If we only consider cell division-coupled mutations and use a mutation rate of 2.5 SNVs per genome per cell division25,26, we can estimate a number of cell divisions per year in SCs of 5.24.

Frame shift mutations and use of alternative reading frames have been described for HRSV (35 – 35 ).

This study provides proof-of-concept for TALEN-mediated in situ correction of an endogenous patient-specific gene mutation and used an unbiased screen for comprehensive TALEN target mapping that will cooperatively facilitate translational application.

These plants were assumed to be homozygous for the soh715 mutation and used for initial mapping of the mutation.

In these cases, the reverse mutation was generated and used as 'wild type' instead.

Besides, some new crossover and mutation operators are developed and used in this work.