Sentence examples for mutations and screening from inspiring English sources

The theoretical grounds underlining the design of such libraries are described, including the mutational load, the ratio of beneficial versus deleterious mutations, and screening capacity.

The use of synthetic oligonucleotides for recombination circumvents any cloning steps and provides maximum flexibility for introducing mutations and screening markers.

First, we employed a motif finder, part of Mod Tools [75], to locate potential HSEs by searching for the 8-nucleotide sequence TTCNNGAA, allowing for a maximum of 4 mutations and screening for 8 bp sequences with a matrix score of at least 70.

In dinoflagellates, the lack of axenic cultures, as well as the difficulty in constructing genetic mutations and screening mutants, has meant that confirming the roles of genes in biosynthetic pathways is currently not possible.

Accumulated evidence indicates that FLT3-ITD functions in approximately 20% of AML as driver mutations, and screening FLT3-ITD could benefit to select the more accurate prognostic criteria and therapeutic strategies.

One of these T4 K10 Δ 39.2 bacteriophages was extensively backcrossed to wild-type T4Do, continuously selecting for loss of the amber mutations and screening by PCR for the simultaneous loss of the 39.2 gene to arrive at the isogenic T4 Δ 39.2 bacteriophage used in this work.

The chapter explores the theoretical basis for each process in directed evolution: mutation, recombination, and screening.

We randomly picked five transformants per mutation and screened them for cell growth and production of active HRP in shake flasks.

This result, combined with the observed reasonable phenotypic penetrance, makes PB transposition an attractive, nonviral approach to validate positionally-cloned gene mutations and screen candidate genes.

Sanger sequencing of PCR products was used to verify the mutations and screen additional patients and controls.

A well-controlled lethal rate is fundamental for effective mutation and screening of the mutants.