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RT-PCR assays for three K-ras gene mutations and one BRAF gene mutation were included in the assay panel along with each corresponding wild type allele assay.
Four minor mutation sites caused three nonsense mutations and one frameshift on the pol or env region (Supplementary Table S5).
The number of patients carrying a TLR4 mutations and one of NOD2 and/or HSP70-2 mutations, was 20 (19%) vs 17% in controls subjects.
The resulting mutational constructs of ALR2 (eight point mutations and one double mutant) were probed for their influence towards ligand selectivity by X-ray structure analysis of the corresponding complexes and isothermal titration calorimetry (ITC).
To determine the role of conserved amino acids in the substrate binding pocket, five single mutations and one double mutation were studied for their effects on substrate specificity.
The protein translocase gene secY was mutated in seven of thirteen evolved isolates, totaling six amino acid mutations and one in-frame deletion.
On chromosome III, whole genome sequencing found four nonsynonymous mutations and one stop-gained mutation closely linked to sam12 ; however, RNAi depletion of these genes did not cause somatic P-granule expression (not shown).
Two types of amino acid mutations and one region in the antibody V segment were identified that are critical for HIV-1 neutralization.
We show that it is possible to introduce two point mutations and one gene insertion in one transformation experiment with a very high efficiency.
Recombinant forms have been constructed by successive addition of restricted mutations and one deletion outside the T cell epitope-containing regions.
Due to the fact that the mutants only differ by point mutations and one of the sequences caries 13 mutations (sequence 7), this sequence is set far apart from the other sequences by most descriptor sets.
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