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Hattersley and colleagues [ 30] posited that the association between low birth weight and subsequent insulin resistance in adulthood is genetically determined, presumably a result of GCK mutations, and introduced the 'fetal insulin' hypothesis.
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Interestingly, a reduction of steric interference and hydrolysis suppression was caused by the Y377F mutation and introduced a synergistic effect to produce malto-oligosaccharides with a higher degree of polymerization than wild-type.
The JG operator is associated with some kind of macro macro-mutation and introduces higher exploratory capabilities, counteracting the effect of elitismacro macro-mutation
The mutant site was introduced by means of two partially overlapping primers containing the mutation and introducing an EcoRI site.
The microdeletion removes exon 5 of the ZNF277 gene, causing a frame-shift mutation and introducing a premature stop codon in exon 7 (NM_021994.2).
Seven different amino acid mutations were designed and introduced to the position 461 of the 1a protein in RNA 1.
Fourteen potentially stabilizing mutations were designed and introduced into scFvD1.3 by site-directed mutagenesis, either individually or in combinations.
The cdk9T212E mutation was constructed and introduced into the genome as described elsewhere for cdk9T212A [20].
Mutation primers are listed in Table S1 in Additional file 1 and introduced mutations are in bold and shown above the mRNA in each figure.
First of all, mutants for FM are generated by applying the mutation operators and introducing only a simple modification each time.
Ron Fouchier of the Erasmus Medical Center in the Netherlands and colleagues started off with H5N1 isolated from an Indonesian patient and introduced mutations to the virus's genes.
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