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Here, we used the CRISPR/Cas9 system to target genomic mutations and insertions.
Topology is interesting because single nucleotide variations (SNV), point mutations, and insertions or deletions (indels) can indirectly impact the expression of a distant gene by rearranging the genetic architecture [66].
Non-conserved missense mutations and insertions that affect the PRNP gene, which encodes the PrP protein, are associated with inherited human prion diseases.
Hence, we restricted our analysis to a more general analysis of the impact of mutations and insertions on the mapping accuracy.
In the current study we have introduced mutations and insertions at only one point.
The mutation data we used included non-synonymous point mutations and insertions and deletions (indels) in coding regions.
Similar(48)
Point mutations and insertion or deletion events (called indels) can be detected.
In the AKAP7 long form splice variants, multiple nucleotide mutations and insertions-deletions have resulted in the concurrent loss of AKAP7γ and formation of AKAP7δ in rats.
The point mutations and insertion mutations were performed with the Stratagene QuikChange mutagenesis kit according to the manufacturer's protocol.
The identification of microinversions can be difficult because point mutations and insertion-deletion events (indels) continue to accumulate after inversions.
These mutations include missense and nonsense substitutions, frame shifts and premature translation termination mutations, deletions and insertions.
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