Sentence examples for mutations and generated from inspiring English sources

However, when we have separated the mutations and generated new alleles that retain only some of them in a wt backbone, we failed to isolate a group of mutations that would be responsible for the toxicity.

All samples were previously tested for CNVs in BRCA1 and BRCA2, and we correctly identified all known mutations and generated no false positive calls in these genes.

Because the dfoxO21 original stock is homozygote lethal but dfoxO null mutants are homozygous viable [24], [30], we recombined dfoxO21 with yw to eliminate second site mutations and generate homozygous viable lines.

The method allows the submission of a number of proteins and mutations and generates a detailed informative document of the prediction and obtained results.

Thus, there is a strong interest in improving our fundamental understanding of how these tumors evolve genomic mutations and generate intratumor heterogeneity that confounds clinical diagnostics and treatment of patients.

International Gene Trap Consortium (IGTC) maintains a database to curate functions of genes across the mouse genome by gene trapping that is a high-throughput approach to introduce insertional mutations and generate loss-of-function alleles in embryonic stem cells [ 28].

One recurrent mutation (c.7544_7545fsdel) accounts for approximately 80% of all NOTCH1 mutations and generates a premature stop codon in the PEST domain (a peptide rich in proline (P), glutamic acid (E), serine (S) and threonine (T), thought to act as a signal for protein degradation [ 42]), which normally limits the intensity and duration of NOTCH1 signaling [ 39].

To investigate further, we tested the canonical signaling strength of existing fz alleles, by recombining them with a strong fz2 mutation and generating loss-of-function clones in the wing margin.

4) Finally, we introduced these four mutations into FLCE and generated recombinant FLCE_mu4.

Molecular recombination is an important evolutionary mechanism that allows deleterious mutations removal and generates allelic diversity in a population.

Notably, both frameshift mutations and substitutions generated premature stop codons in 92% of the cases (Table 2), indicating the particular way in which mucA was mutated.