Sentence examples for mutations and complementation from inspiring English sources

This could be attributable to the fact that they performed the mutations and complementation study in a wsc1 ∆ mid2 ∆ double-mutant that exhibited a severe lysis defect at all growth temperatures in absence of osmotic support.

Mutation and complementation implicate each gene in O-antigen expression but, as their putative sugar nucleotide substrates are not currently available, biochemical characterisation of WbmF, WbmG and WbmH is impractical at the present time.

The strategy involves combining a sequential mutagenesis scheme to reduce library size with structurally stabilizing mutations, chaperone complementation, and reduced temperature of gene expression.

All plants tested were more susceptible than the resistant Kas-1 ecotype and complementation of the atl9 mutation restored the wild-type defense phenotype (data not shown).

Transformants can be selected by complementation of auxotrophic mutations, and the use of antibiotic resistance genes as selectable markers is not required.

Genetic mapping and complementation tests, utilizing the bleach sensitivity phenotype, assigned 27 independent mutations to nine complementation groups.

Alleles of unc-2, unc-36, eat-2, and slo-1 were identified by complementation tests with existing mutations and scored by the visible locomotion and feeding phenotypes of these mutations.

Complementation among the mutations yielded six complementation groups.

As we were not able to identify the other ins mutations by complementation experiments due to high reversion rates, we looked at phenotypic analogy with mutations described in the litterature.

Importantly, such experiments might also allow quantification of the extent to which stabilizing mutations, chaperonin complementation, temperature etc., modify protein neutral networks (those sequences that map to a GFP structure that provides green fluorescence) and hence contribute to an integrated description of how such parameters impact protein evolution.

Three of my postdoctoral fellows (who contributed to the isolation of mutations, complementation testing, their characterization and their molecular identification) were co-first authors when the work was published in Nature, and the work has spawned a multitude of publications and ongoing projects.