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For EGFR mutations analysis, we used the EGFR Scorpions kit (DxS, Manchester, UK), which combines Scorpions amplification refractory mutation system (ARMS) and Scorpions technology, to detect mutations in Real-time Polymerase Chain Reaction (PCR) reactions.
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Two cell lines generated from glioblastoma samples included in our mutational screen were also subjected to the mutation analysis we performed.
The mutation analysis we performed for our motif might have a different outcome when conducted within the folds of a different protein.
For the LAMC2 mutation analysis we sequenced overlapping cDNA fragments spanning the entire open reading frame in each two affected and non-affected sheep.
Furthermore, by mutation analysis we showed that a CRX binding element located within the 134 bp Nxnl1 fragment is necessary for promoter activity, thereby implicating CRX in the cell type-specific regulation of RdCVF expression.
In the full mutation analysis we detected two missense variants and eight intronic polymorphisms.
To simplify mutation analysis, we built a web interface for variant analysis using Delila software as the processing backbone.
Through detailed mutation analysis, we identified three S/T-rich motifs located in the AF2 domain of ER α.
On the basis of microsatellite marker and mutation analysis, we identified eight pedigrees where the younger sib was presymptomatic.
As a positive control for the mutation analysis, we also included genomic DNA prepared from two colon cancer biopsies known to be positive for KRAS mutation.
For mutation analysis, we successfully sequenced DNA from 61 of the 62 sample pairs of cancerous and noncancerous liver tissue, and all control samples.
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