Sentence examples for mutations a two-step from inspiring English sources
To isolate recessive mutations, a two-step breeding scheme was used.
To make RoR or RoR promoter constructs carrying deletions or mutations, a two-step PCR procedure was used as described previously.
The *1 and *10 alleles were identified as the C188 T mutation using a two-step polymerase chain reaction (PCR) analysis as described by Johansson et al (1994).
Hockemeyer and his UC Berkeley colleagues, in collaboration with dermatopathologist Boris Bastian and his colleagues at UCSF, found that immortalization is a two-step process, driven initially by a mutation that turns telomerase on, but at a very low level.
Congratulations to Franzi and Chiba for their publication of "Mutations in the promoter of the telomerase gene TERT contribute to tumorigenesis by a two-step mechanism" in Science.
Chiba K, Lorbeer F, Shain A, McSwiggen D, Schruf E, Oh A, Ryu J, Darzacq X, Bastain B, Hockemeyer D. (2017) Mutations in the promoter of the telomerase gene TERT contribute to tumorigenesis by a two-step mechanism.
Markerless allelic replacements were performed as described previously40 using a two-step procedure based on a bicistronic kan-rpsL cassette called Janus to delete the genes of interest, replace them by fluorescent protein fusion or introduce point mutations.
A two-step approach can help here.
Hearing is a two-step process.
A two-step no-intermediate mechanism.
