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To examine the mutational trend of amino acid composition in MOMP evolution, we used the ompA sequences of prototype strains as baseline.
Considering the above evidenced strong selective pressure acting on this dominant chlamydial antigen, we examined the mutational trend of amino acid gains and losses in MOMP evolution.
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In order to assess how well CMC spectrums estimate the aggregate non-synonymous mutational trends of a single gene, a CMC matrix was applied to seven unrelated genes to compute the most likely point mutations.
In this worldwide survey, we intended to provide a more complete picture of the mutational trends of the C. trachomatis leading multi-subunit vaccine candidate.
We also explored the composition-dependent mutational trends of the tradeoff.
Even with this extremely short time-scale (about six decades), our results clearly evidence the existence of intrinsic mutational trends for MOMP, a crucial antigen, adhesin, and porin.
Analysis of the mutational trends in this region of the α-helix between the 8q24.3 ZNF paralogues should help to clarify likely duplication scenarios and functional, i.e. nucleic acid binding residue, variability.
Resulting compositional tradeoff observed here underlies mutational trends and mutual tuning of the nucleotide and amino acid compositions.
Although the impact of these mutational trends in MOMP evolution is not known, the gain or loss of certain amino acids were already shown to influence the structure of several bacterial porins [12].
If one of the latter is dominant, then it may be possible to monitor functional mutational trends hydropathically, through quantitative trends in solvent-accessible amino-acid specific surface areas [ 11, 12].
Although substitution at residues 530 and 549 of the H protein has a crucial role in host switching [ 10], the observed mutational trends indicate that a geographic signature cannot be ruled out.
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