Both mutational substitutions are located in EF-hand domains, suggesting that these changes affect the interaction between calcium and the regulatory subunit and possibly impact the activation of the catalytic subunit.
Six of the mutational substitutions constructed are pathological mutations found in compound heterozygous FRDA patients (D122Y, G130V, N146K, I154F, W155R and W173G) [25].
a In Curated Kinase Docking, the UniProt ID, residue position, and mutant amino acid should be specified in Kinase and Mutational Substitutions.
In this study, we probed how mutational substitutions of streptococcal pyrogenic exotoxin A (SPEA), a model bacterial superantigen, affect native conformational stability and antigenicity.
Sulphonamide resistance in C. jejuni is also chromosomally mediated through mutational substitution of four amino acid residues in the enzyme dihydropteroate synthetase (DHPS), resulting in a reduced affinity for sulphonamides.
Interactions also enhance the stability of individual domains, which constrains mutational substitution of interacting residues.
The R-dependent component is defined as whatever effect is generated due to mutational differences between leading and lagging strands while the R-independent component is comprised of those factors, mutational or selective, that affect substitutions in the same manner regardless of which strand is being considered.
The mutational analysis revealed that amino acid substitutions in the Upf3 NES impair both nuclear export and the Upf2p-Upf3p interaction, both of which are required for Upf3p to function in NMD.
Thus, we concluded that nonsynonymous nucleotide substitutions in these codons are likely caused by random mutational process after integration of mtAncestor-2 into the nuclear genome and excluded these mutations from our analysis.
This indicates that the strand effects inferrable from substitutions in human and chimp introns are not typical for the long-term mutational regime that has shaped intron primary structure.
