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Recently, mutational studies of FtsY have revealed that site-specific mutations can modulate discrete conformational changes during Ffh·FtsY complex formation [23].
HRM is an effective technique for simple and rapid scanning of TP53 mutations that can markedly reduce the amount of sequencing required in mutational studies of TP53.
Large-scale mutational studies of large cohorts of PDAC (n=100) have not revealed mutations in BMPRIA (Biankin et al, 2012) and studies that specifically mention analysis of BMPRIA in pancreatic cancer have also not revealed mutations (n=31; Jones et al, 2008; Kan et al, 2010).
The mechanism of protein folding has been addressed with mutational studies of folding rates and equilibrium constants.
Herein, we report a 2.9 Å structure of the eArgRS-L-arginine complex (PDB ID: 4OBY) and a series of mutational studies of the enzyme active site.
NMR chemical shift perturbation and mutational studies of DNA binding activity in vitro reveal how Mcm10 uses this unique surface to engage ssDNA.
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We conducted a mutational study of SifA to identify domains and amino acid residues specifically relevant for intracellular replication and SIF formation.
Analysis of these systems and a mutational study of selected candidates revealed that the presence of proline and glycine residues in the loops of β-helical structures greatly enhances the structural stability of the systems.
A mutational study of an HCV subgenomic replicon suggested that the 5'-proximal stem-loop of the HCV RNA is an essential cis-acting RNA element for HCV genome replication [40].
► Mutational study of the ion gate of Mg2+ transporter CorA.
SC and PK were responsible for insilco mutational study of novel gene, dfrA30.
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