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These results provide the basis for future mutational studies for more extensive function characterization of the Mpl sequence-structure relationships.
- Interestingly, previous mutational studies for short-sleeping mutants had already implicated Shaker channels in the regulation of sleep in Drosophila.
This could be achieved by the increase of the catalytic capability of their cyt bc1 complex with Asp272 substituted by Glu272, as indicated by the mutational studies for the cyt bc1 complex from yeast (Wenz et al. 2006) and from R. capsulatus (Gennis et al. 1993).
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In addition to the use of the literature as validation, we conducted a mutational study for validation purposes where dsx null mutants were examined.
For mutational studies of tumour samples, DNA was extracted from macrodissected tumoural paraffin-embedded tissue using the QIAamp Tissue Kit (QIAGEN GMBH, Hilden, Germany) according to the manufacturer's protocol.
Based on the conserved motifs, eight important residues were identified for the mutational studies of FadD13.
Mutational studies of the IZUMO1 JUNO interface by both groups reveal the structural determinants required for binding.
Structural and mutational studies of the CARD domains of Apaf-1 and procaspase-9 have identified the essential motifs for procaspase-9 activation by Apaf-1 [34].
SC and PK were responsible for insilco mutational study of novel gene, dfrA30.
As mentioned, most of the (H, N, C) NMR resonance signals originating from IsdBN1 could be assigned to specific amino acids except for one large contiguous stretch of residues located within the loop 2 region of IsdBN1 including four aromatic residues (F164Y165H166Y167) that, based on our structural analysis and recent mutational studies, are critical for IsdBN1 binding to Hb.
We conducted a mutational study of SifA to identify domains and amino acid residues specifically relevant for intracellular replication and SIF formation.
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