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Mutational exchange of both aspartic acid residues into alanines strongly reduces enzymatic activity [3], [6].
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Mutational analysis showed that exchange of a single amino acid within the peptide ligand caused a total loss of binding activity.
Mutational analyses demonstrated that the exchange of both R416 and W417 significantly destabilized the resulting variants [28] suggesting that they should not be included in library design.
All samples showed wild-type sequence for exon 9 and exon 20, with two exceptions: In one single WBC of patient IB14, we detected a silent mutation (c.3159A > G) near the mutational hotspot of exon 20, and we found a single WBC of patient IB07 harboring a previously not described mutation at codon 1,015 resulting in the exchange of serin to tyrosine.
Later, mutational studies of chlorite dismutases from Dechloromonas aromatica (DaCld) and " Candidatus Nitrospira defluvii" showed that the exchange of those conserved tryptophan residues on the proximal side did not prevent deactivation of Clds.
Mutational analysis of LMP2A signaling motifs showed that activation of the pZ promotor required a functional ITAM as exchange of its tyrosines to phenylalanine residues (Y74, 85 F) abrogated BZLF1 expression.
Exchange of ideas.
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