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16, 23, 39 Mutational analysis for activating mutations is strongly recommended in all advanced GISTs because of their predictive value.
In contrast to mast cell leukemia we found no clear-cut correlation between KIT mutation and MAGE gene expression: mutational analysis for KIT was found in 5/28 available samples and CT7 expression was detected in none and CT10 in 1 of 5 (20%) of these cases (primaries and metastasis).
Mutational analysis for activating KIT and PDGFRA mutations is strongly recommended in KIT-negative tumors and in all advanced GISTs.
Based on this idea, we performed a joint mutational analysis for these genes and our data of NOTCH1-FBXW7 mutation rate was 56.4% of T-ALLs, which is in line with previous reports on childhood T-ALL [ 35, 36].
A mutational analysis for k-Ras was done and the tumour resulted k-Ras mutated.
To show mutational analysis for 11β-hydroxylase deficiency (11β-OHD).
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We sincerely thank Dr. Carmela Migliaccio for mutational analysis of the EPOR gene and Dr. Luciana De Vito for preliminary immunoblotting.
This system has been consistently used for mutational analysis of nuclear proteins to delineate and characterize the NLS [52] and for testing the integrity of the nuclear pore complex permeability barrier [53].
These results recommend paying attention to biased PCR amplification that may lead to misjudgment of the result for mutational analysis of the MECP2 gene.
Methods: For mutational analysis of the IgE-binding epitopes, peptides of 10-14 AAs in length were synthesized on a derivatized cellulose membrane with single or multiple AA substitutions.
OBJECTIVE: To investigate SPOP mutations across diverse cohorts and validate a series of assays employing high-resolution melting (HRM) analysis and Sanger sequencing for mutational analysis of formalin-fixed paraffin-embedded material.
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